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Anti-Semaphorin 3A (SEMA3A) Antibody
Alomone Labs  
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Activity Assay
Cell Culture
Chromatography
Expressing
In Vitro
Labeling
Neutralization
Purification
Recombinant
SDS Page
Staining
Western Blot
A sensory axon repellent secreted from ventral spinal cord explants is neutralized by antibodies raised against collapsin-1 
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Comparison of <t>collapsin-1</t> and collapsin-2 mRNA expression in E6 chick spinal cord. The expression patterns of collapsin-1 (A) and collapsin-2 (B) mRNAs in adjacent fixed and frozen cross sections of a stage 28 (E6) chick spinal cord made at the lumbosacral level and visualized with digoxigenin-labeled RNA probes. A DRG (open arrow) and what will later be the entry point for sensory axons into the dorsal gray matter of the cord (black arrow) are indicated. Scale bar, 200 μm.
A sensory axon repellent secreted from ventral spinal cord explants is neutralized by antibodies raised against collapsin-1 
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Characterization of rabbit anti-chick <t>collapsin-1</t> antibodies. (A) 2 μg of recombinant myc-epitope-tagged collapsin-1 (lanes 1,3,5,6) or recombinant mycepitope-tagged collapsin-2 (lanes 2,4,7) were run on an SDS-PAGE gel and blotted onto nitrocellulose. Lanes 1 and 2 were probed with a monoclonal antibody to the myc epitope, lanes 3 and 4 were probed with anti-collapsin-1 antiserum from rabbit #475, lanes 5 was probed with preimmune serum from rabbit #475, and lanes 6 and 7 were probed with protein-A purified, collapsin-1-depleted antibodies prepared from the same serum. (B) A western blot of collapsin-1 enriched by S-sepharose and WGA chromatography from adult chick brain. 2 brain equivalents of WGA eluate were added per lane. Lane 1 was probed with affinity-purified anti-collapsin-1 antibody from rabbit #475, and lane 2 was probed with affinity-purified preimmune antibody from the same rabbit. The arrowhead indicates the collapsin-1 band in lane 1.
A sensory axon repellent secreted from ventral spinal cord explants is neutralized by antibodies raised against collapsin-1 
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The neutralization of <t>collapsin-1</t> activity by anti-collapsin-1 antibodies in vitro. The relative responsiveness of E7 DRG growth cones when treated with various concentrations of partially purified recombinant collapsin-1 protein preincubated with a fixed amount of rabbit protein-A purified anti-collapsin-1 antibodies (open squares); protein-A purified antibodies from preimmune serum (open triangles); protein-A purified antibodies depleted of anti-collapsin-1 antibodies (open circles); or no antibodies (closed squares). See Methods for details. Error bars indicate 95% confidence limits of the means estimated using the assumption that the data are distributed according to a binomial distribution.
A sensory axon repellent secreted from ventral spinal cord explants is neutralized by antibodies raised against collapsin-1 , 2009 Jul 14
"Comparison of <t>collapsin-1</t> and collapsin-2 mRNA expression in E6 chick spinal cord. The expression patterns of collapsin-1 (A) and collapsin-2 (B) mRNAs in adjacent fixed and frozen cross sections of a stage 28 (E6) chick spinal cord made at the lumbosacral level and visualized with digoxigenin-labeled RNA probes. A DRG (open arrow) and what will later be the entry point for sensory axons into the dorsal gray matter of the cord (black arrow) are indicated. Scale bar, 200 μm. "
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Cell CultureCell Culture50.00%
RecombinantRecombinant50.00%
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